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Varietal/Rootstock bud grafted onto 1 year old rootstock in Autumn. Buds allowed to establish and grow away (12-18 weeks). |
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| "Infected Plant" (IP) placed in Heat Treatment (HT) in early Spring, acclimatised and held at 38 degrees Celcius for 6 to 10 weeks. |
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| Growing tips are removed from heat treated IP and grafted on to actively growing Granny Smith/Pear seedlings. |
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| Once buds have callused and starting to grow away, a heat treated plant (HTP) is established and dormancy is induced by chilling at 5 degrees Celcius for 6 weeks. |
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| HTPs are removed from cold storage, grown under optimal conditions, and first round of pathogen testing is done on actively growing tissue. |
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| A second round of pathogen testing is conducted at least 10 weeks after the first, preferably after a period of dormancy. |
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| If all tests are negative, heat treated plants are returned to the client. |
The entire process takes approx. 18 months
Pathogens tested for:
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Apple mosaic virus (ApMV)
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Applechlorotic leafspot virus (ACLSV)
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Apple stem grooving virus (ASGV)
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Apple stem pitting virus (ASPV)
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Apple rubbery wood MLO.
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